One of the most exciting developments in DAW technology over the past couple of years has been the integration of Stem separation. A once unconceivable concept, stem separation allows for ever deeper levels of audio manipulation. In this post we highlight 5 great uses for this game changing technology.

What is Stem Separation/Splitting?

If you’re new to the concept, stem separation refers to the process of extracting distinct elements, such as vocals, drums, bass, and more, from a mixed audio file. It essentially turns any stereo recording into something close to a multitrack session, making it perfect for working with samples, remixes, or live recordings.

As of now, five DAWs support built-in stem separation. Those are FL Studio, Studio One Pro 7, RipX DAW, Serato Studio and Logic Pro (which just added the ability to separate guitar and piano parts).

If your DAW of choice is not on this list then there are 3rd party solutions that will help you to bring the power and possibilities of stem splitting directly into your projects. Most notably, Serato Sampler has high quality integrated stem separation. And as this works as a VST within your DAW it will enable you to perform most of the techniques mentioned in this guide.

1.Sample Loop Variation

One of the most powerful advantages of stem separation in a DAW is the ability to turn a fully mixed stereo loop into a flexible, multi-layered arrangement. Loops are great for sparking ideas, but they often lack variation. With stem separation, that limitation no longer exists.

By splitting a loop into stems, you effectively convert it into a multitrack session, giving you the same creative freedom you’d have with any original project. Each stem is placed on its own track, often within it’s own group. You can easily drop certain elements out and even automate parameters like volume and panning for smooth dynamic control and impact.

This opens the door to countless arrangement possibilities. You can mute certain elements to create breakdowns, chop up individual parts for fills or stutter effects, or apply a creative effect such as reversing a short section of piano. And who doesn’t love a good reversed piano moment?

With stem separation, your loops are no longer static. They become dynamic building blocks for inventive, evolving compositions.

The only thing that shouldn’t be overlooked are the limitations in terms of audio quality. While the possibilities of stem separation seem like the holy grail in reality they often come with sonic artifacts as well as occasional bleeding of elements. And while some audio seems to take to stem separation better than others, this should always be a consideration.

2. Dynamic Spatial Mixing (3D Audio/Immersive Sound)

One fantastic advantage stem separation offers is the ability to manipulate the stereo field with precision. Instead of being stuck with a flat, pre-mixed sample, you can isolate individual elements like drums, vocals, or keys, and place them exactly where you want in the stereo space. And the great thing about using stem separation to make adjustments to the stereo field is you arent actually removing or isolating any elements so don’t have the sonic artifacts that are often associated with this technology.

Want to pan the drums and percussion hard right while sending melodic elements left? Simply split the stems and pan each one independently. This opens up entirely new ways to shape your mix.

This technique becomes especially powerful when layering additional elements. Mixing is all about carving space for everything to breathe. For example, if your sample contains orchestral elements and you want to add your own strings or brass layers, panning can help prevent clashing and enhance the overall width and depth of the mix.

If you’re working in surround formats like 5.1, stem separation takes on a whole new dimension and can transform a basic stereo recording into an immersive, spatial audio experience.

3. Targeted Effects Application

Stem separation doesn’t just give you control over levels and arrangement. It also opens up the ability to apply effects with pinpoint precision and control. Instead of processing an entire stereo file and risking a muddy or over-processed sound, you can now isolate the specific elements you want and apply your chosen effects to them.

For example, imagine working with a rich, jazzy sample featuring a full band. Applying an effect such as a phaser, reverb, or distortion across the entire mix would sound too much. Instead, with stem separation, you can hone in and apply you effect(s) to the instruments you want while keeping the rhythm section dry and tight.

This level of control is especially useful for creating smooth, expressive transitions or building tension and release within a track. And when you combine these effects with automation things start to get really interesting. Recording a filter sweep on a vocal, or gradually increasing reverb on a guitar stem allows you to unlock a whole new layer of creative potential.

You can even use pitch shifting tools to switch up the scale or key of melodic elements within the audio. Imagine taking a vocal, duplicating it one or two times and pitching each duplication to create unique harmonies, all within a single stereo sample. Combining stem separation with effects inside a DAW offers truly unlimited creative potential. So where could you take it?

4. Audio to MIDI Targeted Extraction

Stem separation doesn’t just give you the ability to isolate and manipulate audio. It also unlocks powerful new MIDI possibilities. Once you’ve extracted individual elements like vocals, basslines, or drum parts from a stereo recording, you can take things a step further by converting those stems into MIDI data.

For example, imagine isolating a piano line from a soulful sample. With audio-to-MIDI conversion, you can transform that performance into editable MIDI notes, letting you re-orchestrate it with your own virtual instruments. You then have the option of layering it with the original piano or completely replacing the part with a new sound, like a synth or string section.

This is equally powerful for drums. Extract the drum pattern from a groove and convert it to MIDI to layer punchier drum samples, reinforce the rhythm with electronic hits, or even trigger FX.

When combined with stem separation, audio-to-MIDI becomes more accurate and creatively useful, since you’re working with cleaner, more isolated material. You’re no longer stuck sampling just the surface of a track. You’re extracting its very DNA to utilise in brand new original ways.

5. DJ Mixes, Mash-Up’s and DJ Edits

And finally, one for all all you DJs, mash-up artists, and edit masters out there. DAWs have long been a playground for crafting intricate mixes and re-edits thanks to their pitch-shifting, warping, and arrangement capabilities. But with stem separation now integrated directly into many DAWs, the creative possibilities have expanded dramatically.

Traditionally, when preparing a DJ mix or mashup, you’d be working with full tracks with the vocals, instruments, and everything else baked into one file. In order to create interesting mixes careful editing would be needed, trimming and rearranging sections or using EQ and filtering to duck unwanted elements. Now, with stem separation, you can isolate and manipulate individual parts such as vocals, drums, basslines and melodies with the click of a mouse, giving you full control over how each track fits into your mix.

Want to filter out everything but the vocal for a breakdown? Done. Need to mute the rhythm section to make way for the next tracks groove? Easy. Want to replace the original drums with a classic breakbeat while keeping the rest of the track intact? Stem separation within a DAW makes all these tasks simple and seamless.

And for mashups, it’s a total game-changer. No longer are you limited to the availability of studio acapella’s or instrumentals. And while sonically these will be superior you will always be restricted to the availability of such studio stems. Now you have the option to extract the vocal from any track and pair it with an entirely different instrumental, genre, or tempo.

Whether you’re extending intros for smoother transitions, breaking down bridges to create tension, or layering your own synths and percussion for added energy, stem separation turns your DAW into a fully-fledged remixing powerhouse.


Remember – RouteNote Create subscriptions start from as little as $2.99. You also get 10 FREE credits to spend on samples along with access to our FREE sample pack bundle when you sign-up!